Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Department of Life Sciences and Biotechnology, South Asian University, Akbar Bhavan, New Delhi, India
Research Article
Uptake of Poly-dispersed Carbon Nanotubes Induce Cytotoxic and Cytostatic Effect on Human B Cell Leukemia Cells
Author(s): Babu Mia, Deepam Pushpam, Sameer Bakhshi and Rajiv K. Saxena*
Uptake of Fluorescence-tagged Acid-functionalized Single-walled Carbon Nanotubes (FAF-SWCNTs) was examined in Peripheral Blood Derived Mononuclear Cells (PBMC) preparations derived from six patients of Acute B-lymphocytic Leukemia (ALL), five patients of Chronic B-lymphocytic Leukemia (CLL), and four healthy control blood donors. Mean percentage of B cells in PBMC samples was 14.08% (healthy controls), 56.50% (ALL), and 88.74% (CLL). PBMCs were incubated with FAF-SWCNTs for 24 h and uptake in CD19+ B cells was examined by flow cytometry. In all cases, significant uptake of FAF-SWCNTs was found in all CD19+ B cells. A subpopulation of B cells (6% in ALL samples, 3% in CLL samples) showed significantly greater uptake of FAF-SWCNTs. Cellular distribution of the FAF-SWCNTs internalized by B cells in different samples was also examined by confocal microscopy. All B cells internalized the F.. View More»
DOI:
10.35248/2161-0495-22.12.504