Journal of Clinical Trials
Open Access
ISSN: 2167-0870
ISSN: 2167-0870
College of Life Sciences, Anhui Normal University, Wuhu, China
Research Article
Validation of Reference Genes for Gene Expression Analysis in Fruit Development of Vaccinium bracteatum Thunb using Quantitative Real-Time PCR
Author(s): Feng He*, Liangxian Gui, Yan Zhang, Bo Zhu, Xiaoping Zhang, Min Shen, Fengying Wan, Lu Yang and Jiaxin Xiao*
Background: Vaccinium bracteatum Thunb. (VBT) is widely distributed in the mountainous areas in eastern and southern China. VBT leaves have great medical value and can be used to stain rice to produce “Wumifan”. Its fruits also contain rich nutrients. However, there has been limited attention to exploring the molecular content of VBT. Previously, we performed RNA-seq on three typical VBT fruits that were at various stages of ripening, although a reliable reference gene was lost in validation.
Results: In this study, we selected ten candidate reference genes based on previous studies and transcriptomics analyses. Subsequently, these genes were evaluated using a combination of methods, including geNorm, NormFinder, and Bestkeeper, with a comprehensive ranking assessment. As a result, we found that the actin2, NADH, and ADK.. View More»
DOI:
10.35248/2167-0870.22.12.501