ISSN: 2376-0354
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Forage Livestock Plant Research Unit, Grazinglands Research Laboratory, USDA-ARS, El Reno, USA
Research
Occurrence and Detection of Chimeral Sectors in Leaf Tissue of Novel Lolium multiflorum x L. arundinaceum Hybrids
Author(s): Bryan Kindiger*
A novel diploid, Lolium multiflorum (Lolium perenne L. subsp. multiflorum (Lam.) Husnot (syn. Lolium multiflorum Lam.) line has been previously observed to induce genome loss and dihaploid generation following hybridization with hexaploid, L. arundinaceum (Schreb.) Darbysh.) (Syn.Festuca arundinacea Schreb.). Early observation of chimeral sectors in these F1 has suggested a form of mitotic chromosome or genome loss was responsible for the recovery of the L. multiflorum or L. arundinaceum dihaploids, and that flow cytometry was a methodology that can be utilized to observe such events. Evaluations of leaf tissue nuclei, .. View More»