Pancreatic Disorders & Therapy
Open Access

Department of Biotechnology, University of Barkatullah, Bhopal, India

Publications

• Research   
  Indigenous Trypsin: An Underpinning Approach in Clinical Medicine
  Author(s): Charu Batav* and Ragini Gothalwal
  
  Background: Single cell suspension culture is the prime requirement for cell culture studies as that in clinical medicine. For disaggregating cells, use of enzyme: trypsin has been the first choice by researchers. Commercially, trypsin is obtained from bovine source which adds to the cost of the experiment. Present investigation highlights on utilization of fish waste derived trypsin as a cell dissociating agent anticipated to reduce environmental footprint. Methods: The current study thus aimed at studying the efficacy of fish waste derived trypsin over commercial counterpart as a cell-dissociating agent. The enzyme isolated from Catla catla visceral waste was purified by Gel filtration chromatography, and characterized by peptide analysis BLAST analysis. The efficacy in terms of cell viability was checked over can.. View More»
  DOI: 10.35248/2165-7092.22.12.238

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