Journal of Chromatography & Separation Techniques
Open Access

Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA

Biography

Dr. Chie Saito Ishii is a researcher in the Department of Small Animal Clinical Sciences at the University of Florida's College of Veterinary Medicine. Her work focuses on analytical chemistry and mass spectrometry applications in veterinary medicine. She has co-authored several publications, including: "Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods," published in the Journal of Chromatography & Separation Techniques in December 2023. LONGDOM "Origin and Function of Tryptophan Metabolites Modulating CD4+ T Cells in Lupus-Prone Mice," published in iScience in 2023. "Investigating the Impact of Oxybenzone-Containing Sunscreen on Coral Health and Reproduction," available on SSRN. For more information on Dr. Ishii's work and publications, you can visit her profile on the Journal of Chromatography & Separation Techniques website. LONGDOM

Publications

• Research Article   
  Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods
  Author(s): Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill
  
  Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries .. View More»
  DOI: 10.35248/2157-7064.23.14.539

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