Immunotherapy: Open Access
Open Access
ISSN: 2471-9552
ISSN: 2471-9552
Institute of Cancer Sciences, University of Manchester, Manchester, UK
Research
Multiplex Immunohistochemistry: The Importance of Staining Order When Producing a Validated Protocol
Author(s): Jihad Syed, Jack Ashton, Jesuchristopher Joseph, Gemma N Jones, Christian Slater, Alan Sharpe, Garry Ashton, William Howat, Richard Byers and Helen K Angell*
Aim: The complexity of multifactorial diseases, such as cancer, poses significant challenges to the development of personalised therapies. Integrated digital histological analysis of tumours provides a better understanding of the immune microenvironment and the prognostic relationship associated with the enumeration and distribution of specific tumour infiltrating lymphocyte (TILs) subpopulations. To this effect multiplex cell labelling , alongside multi-spectral imaging (MSI) is an approach increasingly used to achieve more accurate in-situ TIL phenotyping and quantification. However, these approaches require full validation prior to utilisation , which is the fundamental aim of this study.
Methods: Whole sections and tissue microarrays of lymphocyte-rich tissue were used to develop and validate a multiplex immunofluorescence (IF) protocol .. View More»