Journal of Research and Development
Open Access
ISSN: 2311-3278
+44-77-2385-9429
ISSN: 2311-3278
+44-77-2385-9429
USA
Extended Abstract
Mass Spectrometry 2017: Quantification of Temozolomide in Nonhuman Primate Fluids by Isocratic Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry to Study Brain Tissue Penetration Following Intranasal or Intravenous Delivery – C
Author(s): Cody j Peer
A sensitive and selective ultra-high performance liquid chromatography- Tandem mass spectrometric method was introduced for the quantification of temozolomide (TMZ) in nonhuman primate (NHP) plasma, cerebrospinal fluid (CSF), and brain extracellular fluid (ECF) - microdialysis. Ethyl acetate was to extract the plasma and CSF samples, using theophylline because the internal standard (IS). ECF samples were diluted with acetonitrile prior analysis. TMZ was separated on Waters UPLC® BEH C18 column with an isocratic mobile phase ammonium acetate (10 mM)-0.1% formic.. View More»