Journal of Food: Microbiology, Safety & Hygiene
Open Access
ISSN: 2476-2059
+44 1478 350008
ISSN: 2476-2059
+44 1478 350008
Agriculture and Agri-Food Canada, Lacombe Research Centre, Lacombe, AB, Canada
Research Article
Diversity of Microflora Surviving Decontamination Treatments of Pasteurized Meat Carcasses Assessed Using Cultivation-based and Nested PCR-DGGE Fingerprinting Methods Poblational Study
Author(s): Bassirou Ndoye*, Xianqin Yang, Le Luo Guan, Khalifa Ababacar Sylla, Mamoudou H. Dicko, Ibrahima Ndoye, Alfred S. Traore, Amadou Tidiane Guiro and Colin O. Gill
The diversity of microflora was determined to assess the effectiveness of decontamination treatments on pasteurized meat carcasses at a large meat packing plant in Canada. The main objectives were to characterize the bacterial diversity surviving in the heat-treated and DNase-I-treated samples over the untreated samples of pasteurized meat carcasses. Cultivation based-methods were combined with nested PCR-DGGE fingerprinting methods to quantify low numbers of bacterial survival in each sample. Using the DGGE reference marker, seven genera (Pseudomonas, Staphylococcus, Propionibacterium, Chryseobacterium, Flavobacterium, Ralstonia, Paenibacillus) were detected by both DGGE and cultivation-based method. Three species (Streptococcus salivarius, Micrococcus luteus and Leuconostoc mesenteroides) were exclusively found in pure cultures with cultivation-based method. Over f.. View More»
DOI:
10.35248/2476-2059.21.6.149