Andrology-Open Access
Open Access

Department of Mass Spectrometry Diagnostics and Clinical Diagnostic Applications, University of Massachusetts Amherst, Amherst, USA

Publications

• Research Article   
  Understanding Sample Preparation Workflow: Semi-Automated Preparation of Serum Testosterone for Detection Using LC-MS/MS
  Author(s): Ramisa Fariha, Collin Hill, Mohannad Jabrah, Adam Spooner and Anubhav Tripathi*
  
  High-performance liquid chromatography-tandem mass spectrometry has been pivotal to recent clinical advancements in the detection and monitoring of the progression of different metabolic disorders. Such systems have been used to routinely monitor serum testosterone levels in patients, to both mitigate side effects of different medications, as well as diagnose androgen-associated disorders. The present study develops and validates an LC-MS/MS-based assay for the detection of total serum testosterone from only (100 μL) of sample volume. To increase the high throughput capability of the developed diagnostic kit for clinical use on patient samples, the proposed method was streamlined via semi-automation on a JANUS® G3 Workstation liquid handler. The proposed diagnostic kit showcased high detection precision (CV<5%) as well as sample accuracy (93%-108%), withi.. View More»
  DOI: 10.35248/2167-0250.24.13.329

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