Enzyme Engineering
Open Access
ISSN: 2329-6674
ISSN: 2329-6674
Department of Food Science and Technology, Guru Nanak Dev University, Amritsar, Punjab, India
Research Article
Optimization of Enzyme Aided Pigment Extraction from Pumpkin (CucurbitaMaxima Duch) Using Response Surface Methodology
Author(s): Dalbir Singh Sogi* and Anu Sharma
Present work aimed at optimizing the carotenoids extraction from pumpkin with enzymatic pre-treatment. A central composite design with four independent variables-cellulose/pectinase ratio (0.25 - 1.91w/w); Incubation temperature (35-55 ?C); Incubation time (30-150 min); pH (4-6) gave carotenoids yield of 0.689 to 3.820mg/100g. F-value of 39.07 and p value <0.0001 shows that pigment extraction was adequately described by regression model. Linear coefficients of X1, X2, X4, (enzyme ratio, temperature, pH), quadratic coefficient of X12, X22, X32, X42 (enzyme ratio, temperature, time and pH) and interaction coefficient of X1X2 (enzyme ratio and temperature) and X2X4 (temperature and pH) were also significant (p≤0.05). The second order polynomial model had a non-significant lack of fitness, good value of coefficient of determination (0.973) and adequate scattered plot. Surface graph .. View More»