Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Public Health and Community Medicine, Cairo University, Cairo, Egypt
Dalia Ahmed is currently working as professor in Cairo University. Publications include: Smoking problem among Helwan University students: Practical versus theoretical faculty, Is sdLDL a valuable screening tool for cardiovascular disease in patients with metabolic syndrome?
Research Article
Methylenetetrahydrofolate Reductase C677T Gene Mutation as a Risk Factor for Diabetic Nephropathy
Author(s): Dalia Ahmed*
Background: Methylenetetrahydrofolate Reductase (MTHFR) is a regularly enzyme of homocysteine metabolism.
Point mutation in MTHFR and hyperhomocyteinemia are implicated in the pathogenesis of Diabetic Nephropathy
(DN) in many ethnic groups. The aim of this study is to find if MTHFR C677T polymorphism is a risk factor of DN
in Type 2 Diabetes Mellitus (T2DM) patients.
Subjects and methods: The MTHFR C677T polymorphism was detected in 122 T2DM patients by PCR-RFLP. They
were divided into 3 groups; 75 patients with normoalbuminuria. 33 patients with microalbuminuria and 14 patients
with macroalbuminuria. Seum levels of Homocysteine (HCY) were determined by nephelometry.
Results: Presence of MTHFR C677T allele increase the risk of macroalbuminuria 2.6 folds (p=0.009) in T2DM
patients. The presence of mut.. View More»
DOI:
10.35248/jcclm.21.4.180