Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

CMD, Swagat Hospital & Research Center, Bongaigaon, Assam, India

Publications

• Research Article   
  Effect of different types of in vitro maturation medium (IVM) on maturation rate of non-vitrified and post vitrified-thawed pig follicular oocytes
  Author(s): Krishna Kalita, Deka BC, Biswas RK, Barua PM, Borah P, Dutta DJ and Das SK
  
  Purpose: To study the effect of different types of In vitro maturation medium on cumulus cell expansion and nuclear maturation rate of non-vitrified and post vitrified-thawed porcine follicular oocytes. Methods: Three different In vitro Maturation (IVM) media (IVM-I contained only hormones and growth factors, IVM-II contained porcine follicular fluid along with hormones and growth factors and IVM-III contained porcine follicular fluid, foetal bovine serum and growth factors without any hormone) were prepared using modified tissue culture medium (TCM-199) as a base medium for the study. Porcine oocytes were collected and IVM in above media was done for 48 h. Results: In case of non-vitrified and vitrified oocytes the rate of cumulus cells expansion did not differ significantly,.. View More»
  

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