Journal of Food: Microbiology, Safety & Hygiene
Open Access
ISSN: 2476-2059
+44 1478 350008
ISSN: 2476-2059
+44 1478 350008
Beijing, China
Research Article
Rapid Detection of Cronobacter and Salmonella in Powdered Infant Formula and Related Matrices Using Loop-Mediated Isothermal Amplification (LAMP)-Bioluminescent Assays as Compared to the Guobiao Standards (GB) Method.
Author(s): Yan Huang, Raj Rajagopal*, Jianwei Huo, Zhiyong Dai, Chenyan Niu, Xiqing Wang, Can Yi, Jichao Liu, Jun Zhou, Feng Liu and Qing Tao
Cronobacter and Salmonella have emerged as major pathogens of concern in Powdered Infant Formula (PIF). China’s PIF industry is growing, and consumers are concerned about the potential risk of these pathogens in PIF. The PIF producers need rapid, easy to use and specific detection of Cronobacter and Salmonella for monitoring of raw materials, process environment and finished products for implementing effective control measures to prevent contamination. The objective of this study was to determine the specificity and sensitivity of LAMP assays compared to the Chinese National Food Safety (Guobiao, GB) Standards 4789.4-2016 (Salmonella) and GB 4789.40-2016 (Cronobacter) detection in raw materials (milk powder, whey powder, minerals, lutein, nucleotide, arachidonic acid powder, docasahexaenoic acid), process environment samples and finished products (neon.. View More»