Journal of Clinical Trials
Open Access
ISSN: 2167-0870
ISSN: 2167-0870
Laboratory of Nitric Oxide and Oxidative Stress, Federal University of Sao Paulo, Sao Paulo, Brazil
Research Article
Açai (Euterpe oleracea Mart) Modulates Oxidative Stress and Inflammation by Nf-Κb Inactivation and Nrf2 Up-Regulation in Experimental Diabetes
Author(s): Deyse Yorgos de Lima*, Adelson Marçal Rodrigues, Margaret Gori Mouro, Elias Jorge Muniz Seif, Giovana Rita Punaro* and Elisa Mieko Suemitsu Higa*
To evaluate the effects of Açai Extract (EA) on oxidative stress and inflammation induced by high glucose in cultured Mouse
immortalized Mesangial Cells (MiMC) and diabetic rats. MiMC cell viability and proliferation were determined by MTT.
Extracellular and intracellular Nitric Oxide (NO) and intracellular ROS were also measured. The cell proteins were extracted
for analysis of catalase, Nrf2, p-Nrf2, SOD-1, SOD-2, iNOS, NF-κB, p-NF-κB and TNF-α expression. Male, adult Wistar rats
were distributed into 3 groups: control (CTL) and diabetic (DM) rats that received water and DMEA and received 1 mL/day EA (200 mg/kg) via gavage for 8 consecutive weeks. After treatment with EA, metabolic profile, renal function and Thio Barbituric Acid Reactives Substances (TBARS) levels were evaluated, and kidneys were collected for qualitative histological
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DOI:
10.35248/2167-0870.22.S17.001