Journal of Clinical and Cellular Immunology
Open Access
ISSN: 2155-9899
ISSN: 2155-9899
Department of Pediatrics, Saint Peters University Hospital, 254 Easton Ave. New Brunswick, NJ, 08901, United States
Research
Circulating MicroRNA in Autism Spectrum Disorders as a Possible Biomarker of Neuroinflammation: Association with Comorbid Conditions and Monocyte Cytokine Profiles
Author(s): Harumi Jyonouchi* and Lee Geng
Autism Spectrum Disorders (ASD) is characterized by frequent comorbid conditions. Previously, we reported changes in circulating levels of microRNA (miRNA) determined using high throughput sequencing, which were dependent on monocyte cytokine profiles. This study assessed how levels of 7 miRNAs selected based on our previous results, change in association with both comorbid conditions and monocyte cytokine profiles. Circulating levels of miRNAs were measured by quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR) in 130 ASD and 50 non-ASD subjects. miRNA levels were negatively correlated with the production of monocyte cytokines (TNF-α, IL- 6, IL-1ß, and IL-10) in ASD subjects without sleep or seizure disorders, but not in ASD subjects with seizure/sleep disorders or in non-ASD controls. This was most evident between levels of miR-320b, miR-423-5p, miR-37.. View More»
DOI:
10.35248/2155-9899.22.13.666