International Journal of Physical Medicine & Rehabilitation
Open Access

Department of Cardiovascular Research, University of Virginia, Charlottesville, Virginia, United States

Publications

• Mini Review   
  Employing the CRISPR-Cas System for Clonal Hematopoiesis Research
  Author(s): Hayato Ogawa, Soichi Sano* and Kenneth Walsh
  
  Clonal hematopoiesis is a state in which substantial fraction of hematopoietic stem cells acquire mutations in specific driver genes and expand in the absence of an overt hematological malignancy. Recent clinical studies have shown that clonal hematopoiesis increases likelihood of hematological malignancy and cardiovascular disease. While clinical studies have identified countless candidate driver genes associated with clonal hematopoiesis, experimental studies are required to evaluate causal and mechanistic relationships with disease processes. This task is technically difficult and expensive to achieve with traditional genetically engineered mice. The versatility and programmability of CRISPR-Cas system enables investigators to evaluate the pathogenesis of each mutation in experimental systems. Technical refinements have enabled gene editing in a cell type specific manner and at a s.. View More»
  DOI: 10.35248/2329-9096.21.9.582

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