Journal of Clinical Trials
Open Access
ISSN: 2167-0870
ISSN: 2167-0870
Department of Pharmacology, People’s Hospital of Fengjie, Fengjie, China
Research Article
Effect of Tuft-IL-25-ILC2 Pathway on Mouse Small Intestine Stimulated by Trichinella spiralis ES Antigen
Author(s): Bai Jie*, HE Ling and Napisha Jureti
Objective: To explore the regulatory effect of Trichinella spiralis ES antigen on the intestinal immune function, we observed the immune response of mouse small intestine to this antigen by quantifying the changes in the related cytokines of Tuft-IL-25-ILC2 pathway.
Methods: A total of 30 BALB/c female mice were randomly divided into three groups: control group, Trichinella Excretion-Secretion (ES) antigen stimulation group, and IL-25 blocking group. The mice in the control group were injected intraperitoneally with PBS; those in the ES antigen stimulation group were injected intraperitoneally with ES antigen once per day for 7 consecutive days; and those in the IL-25 blocking group were injected intraperitoneally, first with anti-mouse IL-25 monoclonal antibody and 3 days later with ES antigen. Alcian blue-nuclear fast red.. View More»
DOI:
10.35248/2167-0870.23.S21.015