Andrology-Open Access
Open Access

Department of Andrology, Eastern Virginia Medical School, New York, USA

Publications

• Research Article   
  Lifestyle Factors and Laboratory Sperm Processing Techniques Correlation with Sperm DNA Fragmentation Index, Oxidative Stress Adducts and High DNA Stainability
  Author(s): Alicia Lennea Broussard*, Benjamin Leader, Edna Tirado, Helena Russell, Hind Beydoun, Robert Colver, Laura Reuter, Bradford Bopp, Matthew Will, Erica Anspach Will and Glen Adaniya
  
  Purpose: To determine correlation between lifestyle risk factors and sperm quality. Methods: Patients (n=133) who consented for the study completed a lifestyle questionnaire. An aliquot of sperm was frozen at three different time points. Preparation methods for 30 semen analysis were compared: ZyMōt Sperm Separation Device (DxNow), Isolate gradient (Irvine), SpermGrad gradient (Vitrolife), and each gradient was followed by swim-up (SU), Isolate+SU and Spermgrad+SU. All samples were analyzed using the Sperm DNA Fragmentation Assay (acridine orange/flow cytometry SDFA™). Analysis included DNA Fragmentation Index (DFI), Oxidative Stress Adducts (OSA) and High DNA Stainability (HDS). Statistical analysis was performed using JMP (SAS 2018) and P<0.05 was considered statistically significant. Results: The neat.. View More»
  DOI: 10.35248/2167-0250.23.12.292

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