Chemotherapy: Open Access
Open Access
ISSN: 2167-7700
ISSN: 2167-7700
Dr, Department of Hematology, Shanghai Tenth People's Hospital, Tongji University School of Medicine, 301 Yanchang Road, Shanghai, 200072, China
Research Article
CircDPY19L1P1/miR-130a-3p/PNPLA6 Axis: Regulating the Cell Differentiation in Acute Myeloid Leukemia (AML)
Author(s): Ningze Xu, Ruye Ma, Huili Zhai and Houcai Wang*
Objective: Acute Myeloid Leukemia (AML) is one of the most common aggressive hematological malignancies.
circDPY19L1P1 have been demonstrated to present upregulation in AML patients. We attempted to elucidate
molecular mechanism of circDPY19L1P1 underlying AML cell malignancy.
Methods: RT-qPCR evaluated circDPY19L1P1 level in AML bone marrow specimens and cells. RNase R digestion
assay and actinomycin D evaluated circDPY19L1P1 circular characteristics in AML cells. FISH determined
circDPY19L1P1 subcellular distribution in AML cells. Loss-of-function assays clarified circDPY19L1P1 role in AML
cell behaviors. Bioinformatics and mechanism experiments assessed association of circDPY19L1P1 or PNPLA6
with miR-130a-3p in AML cells. Rescue assays assessed regulatory function of PNPLA6 in circDPY19L1P1-meidated
AML cellular phenoty.. View More»
DOI:
10.35248/2167-7700.23.11.172