Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Ophthalmology, Affiliated Hospital of Yunnan University, Yunnan Eye Institute, Kunming, Yunnan, China
Research Article
TNFSF15 Inhibits Progression of Diabetic Retinopathy by Blocking Pyroptosis via Interacting with GSDME
Author(s): Hongkun Zhao, Yating Dai, Yunqin Li, Juanjuan Li* and Hua Li*
Diabetic retinopathy is a common microvascular complication of diabetes and a leading cause of blindness. Pyroptosis
has emerged as a mechanism of cell death involved in diabetic retinopathy pathology. This study explored the role
of Gasdermin E (GSDME)-mediated pyroptosis and its regulation by TNFSF15 in diabetic retinopathy. We found
GSDME was upregulated in the progression of diabetic retinopathy. High glucose promoted GSDME-induced
pyroptosis in retinal endothelial cells and retinal pigment epithelial cells, attributable to activation of caspase-3
which cleaves GSDME to generate the pyroptosis-executing N-terminal fragment. TNFSF15 was identified as a
binding partner and inhibitor of GSDME-mediated pyroptosis. TNFSF15 expression was increased by high glucose
but suppressed by the caspase-3 activator Raptinal. Moreover, TNFSF15 protein inhibited high glucose an.. View More»
DOI:
10.35248/2736-6588.24.7.282