Journal of Molecular Imaging & Dynamics
Open Access
ISSN: 2155-9937
ISSN: 2155-9937
Department of Chemistry, Connecticut College, New London,, USA
Perspective
Migration of leukocytes is controlled by actin flow direction
Author(s): Huta B,*
The integrin lymphocyte function-associated antigen-1 (LFA-1, L2) is involved in a variety of sticky contacts, including antigen recognition, vascular emigration, and leukocyte movement within tissues. The extended-open conformation of integrin ectodomains binds ligand with 1,000-fold higher affinity than the bent-closed and extended-closed conformations. The binding of LFA-1 to ICAM ligands by the I domain in the integrin head is conveyed to the actin cytoskeleton through the -subunit leg, transmembrane, and cytoplasmic domains via adaptors like talins and kindlins that bind particular locations in the -subunit cytoplasmic domain. Measurements of traction force on substrates, as well as more detailed measurements of force within ligands and cytoskeletal components, have suggested that integrins transmit force between extracellular ligands and the actin cytoskeleton, as discussed. In .. View More»