Applied Microbiology: Open Access
Open Access
ISSN: 2471-9315
+44 1300 500008
ISSN: 2471-9315
+44 1300 500008
Department of Microbiology, Faculty of Science, University of Kelaniya, Kelaniya, GQ 11600, Sri Lanka
Original Article
Effect of Temperature, pH, Carbon and Nitrogen Sources on Extracellular Protease Production by Four Geobacillus Species Isolated from Maha Oya
Geothermal Springs in Sri Lanka
Author(s): DMSU Dissanayaka and IVN Rathnayake*
Thermophilic bacteria are biotechnologically important group of microorganisms due to their capability to produce a variety of thermostable enzymes, such as proteases. The aim of the present study was the optimization of protease production by thermophilic bacteria isolated from hot springs (54°C-55.5°C) in Maha Oya, Sri Lanka. Four isolates of thermophilic bacteria that belong to the genera Geobacillus, which were previously been isolated and identified, were used in the present investigation to study their protease production. Among the four isolates, Geobacillus toebii (DMBUK 107191) reached a maximum protein concentration with a level of 858 μg/mL and Geobacillus kaustophilus (DMBUK 107161) showed maximum protease activity of 2.232 units/mL (55°C, pH 7). Studies on the result of temperature and pH on protease activity dis.. View More»