Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Department of Ophthalmology and Neuroscience, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA
Research Article
A Dual-Staining Method to Distinguish Retinal Vessels in Oxygen- Induced Retinopathy (OIR) Model
Author(s): Ling Xu, Ping Hu, Ye Zhang, Jinyi Long, Xue Wu, Yuhong Ye, Ning Cui, Fei Sun, Xinmin Lu*, Jikui Shen* and Da Long*
Purpose: The purpose of this study was to improve a time-saving, cost-effective, and labor-saving method to distinguish the retinal structural vessels, Neovascularization (NV), and Non-Perfusion (NP) areas in Oxygen-Induced Retinopathy (OIR) mice.
Methods: C57BL/6J mice were exposed to hyperoxic conditions (75% ± 2% O2) from Postnatal day 7 (P7) to P12 and then returned to room air to cause maximal retinal Neovascularization (NV). After Retinas were isolated at P17, the retinal whole mounts were successively stained with Griffonia Simplicifolia Lectin (GSL) IB4-594 and GSL IB4-FITC using time- and concentration-controlling staining methods. This improved method only used two staining mediums and no other blocking mediums were required. The images were acquired by fluorescence microscopy and merged by Adobe Photosho.. View More»
DOI:
10.35248/2155-9570.23.14.957