Medicinal & Aromatic Plants
Open Access
ISSN: 2167-0412
+44 1300 500008
ISSN: 2167-0412
+44 1300 500008
Drug Research and Production Unit, Faculty of Pharmacy, Obafemi Awolowo University, Ile-Ife, Nigeria
Research Article
In vitro Antisickling and Sub Chronic Toxicity Studies of the Ethanol Extract of Cnidoscolus aconitifolius in Wistar Rats
Author(s): Mojisola C. Cyril-Olutayo*, Norah O. Akinola, Temitope A. Adeyemo, Idris A. Oyemitan, Ronald A.Bejide and Joseph M. Agbedahunsi
Objective: The current study investigated the mechanism(s) of action of the antisickling property of Cnidiscolus
aconitifolius ethanol extract and determined its histopathological effects on the liver, kidney and spleen of Wistar
rats.
Methods: The antisickling properties of C. aconitifolius (CA) ethanol extract was tested at different concentrations,
using the inhibitory and reversal models. Its effect on the density and mean cell volume of Hb S red blood cells
was evaluated using the discontinuous density gradient assay. Ciklavit®, an antisickling nutritional supplement was
employed as positive while phosphate buffered saline (pH 7.0) was the negative control. Changes in percentage
Red Cell Count (RBC) and Mean Cell Volume (MCV) in each density fraction were determined. Quantification
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DOI:
10.35248/2167-0412.20.9.344