Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Belcorp Research and Development, Tocancipá, Colombia
Juan is currently working in Belcorp Research and Development, Tocancipa in Colombia.
Research Article
HPLC Method Validation for the Quantitative Fast Determination of 8 Organic Uv-Filters in Products with a Level of Sun Protection Factor Emulsion-Type
Author(s): Juan David Marín Arevalo, Elizabeth Claros Ceballos, Alejandra Patricia Guzmán Carantón and Johnbrynner García Galvis*
A fastHigh Performance Liquid Chromatography (HPLC) method was developed and validated for the simultaneous quantitative determination of phenylbenzimidazole sulfonic acid (PBMSA), benzophenone-3 (BZ3), diethylamino hydroxybenzoyl hexyl benzoate (DHHB), octocrylene (OC), butyl methoxydibenzoylmethane (BMBM), ethylhexyl methoxycinnamate (EMC), ethylhexyl salicylate (ES), and homosalate (HS) which are eightorganic UV-filters of the most commonly used in cosmetics and sunscreens. A solution pH 2.0 of phosphate buffer, methanol, and acetonitrile in proportions 15:3:82 respectively as mobile phase was used in isocratic conditions, combined with a Purospher Star ® Performance RP-18e column and eight minutes of analysis time. The proposed method was validated following the recommendations of International Council on Harmonization (ICH) and the Food and Drug Administration (FDA) guideline.. View More»
DOI:
10.35248/2157-7064.21.12.440