Disease Intervention and Prevention, Texas Biomedical Research Institute, San Antonio, TX, 78227, USA
Research Article
Rapid and Efficient Inactivation of SARS-CoV-2 from Surfaces using UVC Light Emitting Diode Device
Author(s): Varun Dwivedi, Jun-Gyu Park, Stephen Grenon, Nicholas Medendorp, Cory Hallam, Jordi B. Torrelles, Luis Martinez-Sobrido and Viraj Kulkarni*
Efforts are underway to develop countermeasures to prevent the environmental spread of COVID-19 pandemic caused by SARS-CoV-2. Physical decontamination methods like Ultraviolet radiation has shown to be promising. Here, we describe a novel device emitting Ultra Violet C radiation (UVC), called NuvaWave™, to rapidly and efficiently inactivate SARS-CoV-2. SARS-CoV-2 was dried on a chambered glass slides and introduced in a NuvaWave robotic testing unit. The robot simulated waving NuvaWave over the virus at a pre-determined UVC radiation dose of 1, 2, 4 and 8 seconds. Post-UVC exposure, virus was recovered and tittered by plaque assay in Vero E6 cells. We observed that relative control (no UVC exposure), exposure of the virus to UVC for one or two seconds resulted in a >2.9 and 3.8 log10 reduction in viral titers, respectively. Exposure of the virus to UVC for four or eight seco.. View More»
DOI:
10.35248/2329-8731.21.9.222