ISSN: 2157-7064
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Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Florida, Gainesville, FL 32608, USA
Dr. Karen C. Scott is a faculty member in the Department of Small Animal Clinical Sciences at the University of Florida's College of Veterinary Medicine. She holds a Ph.D. in Poultry Science (nutrition) from The Ohio State University, where she also completed her M.S. in the same field. Her undergraduate studies in Animal Science were completed at The Ohio State University, following an Associate of Science in Livestock Production from Mount San Antonio College. UF VET MED Dr. Scott's research focuses on animal nutrition, particularly in companion animals. She has co-authored several publications, including: "Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods," which discusses a method to measure purine levels in dog foods. LONGDOM "Body Condition of Feral Cats and the Effect of Neutering," a study examining how neutering affects the body condition of feral cats. MADDIE'S FUND VETERINARY MEDICINE "Comparison of the Guaranteed Analysis with the Measured Nutrient Composition of Commercial Pet Foods," which evaluates the accuracy of nutrient content claims on pet food labels. RESEARCHGATE In 2019, Dr. Scott held the position of Biological Scientist 4 at the University of Florida, with a reported salary of $45,985. OPENPAYROLLS For more information on Dr. Scott's work and publications, you can visit her profile on the University of Florida's College of Veterinary Medicine website. UF VET MED
Research Article
Modification of a Targeted Metabolomics Method Using Hydrophilic Interaction Liquid Chromatography-High Resolution Mass Spectrometry to Measure Nine Purines in Commercial Dog Foods
Author(s): Chie Saito Ishii*, Karen C Scott, Carolina Abrahan, Joy G. Cagmat, Taylor A. Harmon, Yann Queau, Vincent Biourge, Timothy J. Garrett and Richard C Hill
Urate is the third most common component of canine uroliths. Dogs with a genetic mutation or hepatic dysfunction are prone to forming urate uroliths because they excrete more poorly soluble urate as the end product of purine metabolism. Dietary purine restriction has been reported to reduce urinary urate concentrations in dogs, but the amount and types of purine in commercial diets have not been well documented. A previous targeted metabolomics method was modified to measure eleven purine metabolites in commercial dog foods. The method utilized hydrophilic interaction liquid chromatography-high-resolution tandem mass spectrometry with stable isotope-labeled internal standards. The within-day and between-day coefficient of variation of each purine metabolite concentration in quality control dog food samples averaged 6% (range: 4%-13%) and 15% (range: 11%-19%), respectively. Recoveries .. View More»
DOI:
10.35248/2157-7064.23.14.539