Journal of Chromatography & Separation Techniques
Open Access
ISSN: 2157-7064
+44 1300 500008
ISSN: 2157-7064
+44 1300 500008
Department of Microbiology, Institute of Food Science and Technology, Dhaka, Bangladesh
Research Article
Decolorizing Stevia rebaudiana (Bert.) Leaf Extracts with Activated Charcoal and Qualitative Analysis of Stevioside Using Chromatographic Methods
Author(s): Mohammad Nazrul Islam Bhuiyan*, Kazi Asma Ahmed Shamima, Meher Nahid, Sadia Afrin, Mohammad Amirul Hoque, Mohammad Khabir Uddin Sarker, Mohammad Abdus Satter Miah and Mohammad Aftab Ali Shaikh
The Stevia rebaudiana (Bert.) leaves are natural low-calorie sweeteners used in many products, including foods, drinks, medicines, cosmetics, and more. This study aimed to use activated charcoal to remove color, purify stevioside from Stevia leaves, and utilize the chromatographic method to confirm its identity. We developed a novel eco-friendly method to decolorize and purify stevioside from Stevia leaf. The yield of stevioside was calculated by weighing the crystallized sample following freeze-drying. The average yield is 8.13%. Ultraviolet-Visible (UV-Vis) and Fourier Transform Infrared (FT-IR) spectroscopy were performed to confirm the identification. This purified sample showed a wavelength region of 4000–650 cm-1 in the FT-IR and a UV-Vis spectrum at 206.49 nm, which referred to it as a stevioside. In HPLC, the purified samp.. View More»
DOI:
10.35248/2157-7064.23.14.513