Journal of Clinical Chemistry and Laboratory Medicine
Open Access
ISSN: 2736-6588
ISSN: 2736-6588
Department of Medicine, Laboratory of Toxicological Genetics and Antitumoral Assessment (TOXGEN), Federal University of Piaui, 64607-670, Picos-PI, Brazil
Research Article
Antitumoral and Chemoprotective Evaluation of Isolated Bromelain and in Combination with Doxorubicin
Author(s): Athanara Alves de Sousa*, Taline Alves Nobre, Jorddam Almondes Martins, Kennyana Luz Miranda, Ana Rafaela Silva Pereira, Marlene Gomes de Farias, Ingredy Lopes dos Santos, Felipe Pantoja Mesquita, Raquel Carvalho Montenegro, Jose Roberto Ferreira Junior, Nicole Debia, Felipe Cavalcanti Carneiro da Silva, Juan Carlos Ramos Goncalves, Anderson Nogueira Mendes, Paulo Michel Pinheiro Ferreira and Joao Marcelo de Castro e Sousa
Use of dietary components as antineoplastic agents has been highlighted due to their high biological activity against tumor cells, chemo preventive effects and low toxicity. The present study evaluated antitumor effects and chemo protective potential of Bromelain (BL) alone and in combination with Doxorubicin (DOX) using in vitro tests: Alamar blue in the lines: AGP01, SKMEL103 and CAL27; MTT assay (3-(4, 5-dimethylthiazolyl-2)-2, 5-diphenyltetrazolium bromide), fluorescent labelling in murine Sarcoma 180 (S180) and comet assay in human lymphocytes. Cytotoxic effects of BL were noted with IC50 (μg/mL) of 124.80 (AGP01), 91.81 (SKMEL103), 95.75 (CAL27) and 25.27 (S180). When incubated in a BL+DOX combination, they demonstrated combination indices ranging from synergistic to antagonistic. In the cell death mechanism at S180, an increase in the number of cells unde.. View More»
DOI:
10.35248/2736-6588.23.7.281