Journal of Chromatography & Separation Techniques
Open Access

Department of Pharmacy, Kohat University of Science and Technology, KPK, Pakistan

Biography

Khan SA is currently working in the Department of Pharmacy in the Kohat University of Science and Technology located at KPK, Pakistan.

Publications

• Research Article   
  Simultaneous Determination of Amoxicillin, Clarithromycin and Esomeprazole in Mice Plasma after Oral Administration by Reverse Phase HPLC Method
  Author(s): Mustafa A, Ali H*, Bibi R, Hassan A, Khan S and Khan SA
  
  A simple and precise reverse phase high-performance liquid chromatography (RP-HPLC) method for simultaneous determination of amoxicillin, clarithromycin and esomeprazole in mice plasma after an oral administration was developed and validated. An isocratic elution was achieved on C18 column with a mobile phase containing buffer, potassium di hydrogen phosphate (KH2PO4 ) 0.05 M at pH 5 and methanol (60:40 v/v) at a flow rate of 1 mL/min, injection volume was 10 µL and UV detection was kept at 205 nm. Linearity was in the range of 0.5-100 µg/mL for amoxicillin, 50-1000 µg/mL for clarithromycin and 0.1-100 µg/mL for esomeprazole. Limit of detection (LOD) and Limit of quantification (LOQ) were 0.26 µg/mL and 0.79 µg/mL for amoxicillin, 8.97 µg/mL and 27.20 µg/mL for clarithromycin, 0.13 µg/mL and 0.39 µg/mL for esomeprazole respec.. View More»
  DOI: 10.35248/2157-7064.21.12.439

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