Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Department of Clinical Medicine, Jiangsu University, Jiangsu, China
Research Article
Cassia Polysaccharide Regulates the Effect of PAX6 Low Expression on Myopia through the Wnt/?-Catenin Pathway
Author(s): Luo Jincheng*, Li Doudou, Li Peixuan, Li Jialin, Sun Xuewei, Wang Xue and Qiu Hongbin
Objective: Aimed to explore the protective effects of cassia polysaccharides on myopia by examining their influence on Adult Retinal Pigment Epithelium-19 (ARPE-19) cells with reduced Paired Box Protein (PAX6) expression.
Methods: ARPE-19 cell line with diminished PAX6 expression was established using a lentiviral approach and addition of XAV-939, an inhibitor of the Wnt/β-catenin. We assessed the expression of genes and proteins involved in the Wnt family member 2 (Wnt/β-catenin, scleral remodeling and cell cycle regulation following treatment with cassia polysaccharides. Gene and protein expression were quantified using Reverse Transcription Polymerase Chain Reaction (RT-PCR) and western blot analyses, respectively. Additionally, the migratory capabilities of these cells were evaluated using a scratch ass.. View More»