Journal of Proteomics & Bioinformatics
Open Access
ISSN: 0974-276X
ISSN: 0974-276X
Department of Surgery, University of Michigan Medical Center, Ann Arbor, MI 48109, United States
Research Article
Large Scale Screening and Quantitative Analysis of Site-Specific N-Glycopeptides from Human Serum in Early Alzheimer’s Disease Using LC-HCD-PRM-MS
Author(s): Lingyun Pan, Yu Lin, Jianhui Zhu, Jie Zhang, Zhijing Tan and David M. Lubman*
Glycopeptide analysis by mass spectrometry may provide an important opportunity in discovery of biomarkers
to aid in early detection of Alzheimer’s Disease (AD). In this work, we have used a NanoLC-Stepped-HCD-DDAMS/
MS platform and a NanoLC-Stepped-HCD-PRM-MS platform for large-scale screening and quantification
of novel N-glycopeptide biomarkers for early detection of AD in patient serum. N-glycopeptides were retrieved
from 10 μL of serum in patients with mild cognitive impairment (MCI, a prodromal phase of AD) and normal
controls, respectively, after trypsin digestion, glycopeptide enrichment, fractionation, and NanoLC-Stepped-
HCD-DDA-MS/MS or NanoLC-Stepped-HCD-PRM-MS analysis. Using a combination of Byonic, Byologic
and Skyline softwares, we were able to accomplish both identification and label-free quantitation of site-specific
N-glycopeptides b.. View More»
DOI:
10.35248/0974-276X.22.15.587