Journal of Molecular Imaging & Dynamics
Open Access

Department of Neurosciences, School of Medicine, Case Western Reserve University, Cleveland, USA

Publications

• Opinion Article   
  Molecular Tracking and Localization of Bio Orthogonally Labeled Plasma Membrane Proteins in Live Cells
  Author(s): Logan Brennan*
  
  Genetic Code Expansion (GCE) in combination with orthogonal labelling provides an elegant method for labelling proteins in live mammalian cells with organic fluorescent dyes (Fl-dyes). A Non-Canonical Amino Acid (ncAA) with a functional group is incorporated into the protein sequence in response to an in-frame amber stop codon (TAG) via an orthogonal tRNA/tRNA-synthetase pair (reviewed in Labeling is then carried out by a rapid and specific bio orthogonal reaction between the functional group and a Fl-dye in GCE-based labelling. As a result, the protein of interest is fluorescently labelled on a specific residue, eliminating the requirement for protein tag fusions (i.e. fluorescent proteins or self-labelling proteins) in live cell applications... View More»
  

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