Journal of Clinical Trials
Open Access

Department of Microbiology Program, College of Science, Technology, Engineering and Mathematics (C-STEM),Alabama State University, Montgomery, Alabama, USA

Publications

• Research Article   
  The ℇ34 Phage Tailspike Protein : An In vitro Characterization, Structure Prediction, Potential Interaction withS. newingtonLPS and Cytotoxicity Assessment to Animal Cell Line
  Author(s): Joseph A Ayariga*, Logan Gildea, Hongzhuan Wu and Robert Villafane
  
  The ?34 phage is a member of the podoviridae family of phages, (short non-contractile tailed bacteriophages) that uses Salmonella newington as its host. This phage initiates the infection of its host via a specific interaction between the lipopolysaccharides (LPS) of the bacterial host and its tailspike protein (TSP). The ?34 TSP is structurally similar and functionally equivalent to the P22 phage whose TSP has been well characterized and electron micrographs of both phages appear indistinguishable. The crystal structure of P22 phage TSP in complex with the O-antigen of S. typhimurium has been determined; and the active site of the TSP demonstrated to be the residues Asp392, Asp395 and Glu359 of the receptor binding domain. In another phage called E15, a phylogenetic relative of ?34 phage, a short polysaccharide consisting of α-Gal-Man-Rha repeating un.. View More»
  

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