Journal of Clinical and Experimental Ophthalmology
Open Access
ISSN: 2155-9570
ISSN: 2155-9570
Department of Surgical Sciences, Uppsala University, Uppsala, Sweden
Research Article
Exposure to Subthreshold Dose of UVR-B Does Not Induce Apoptosis in the Rat Lens In Vivo within First Twenty Four Hours
Author(s): Konstantin Galichanin*, Lovisa Falkman and Zhaohua Yu
Purpose: The aim of this study is to investigate the time evolution of active caspase 3 within first 24 hours in the rat lens after in vivo exposure to subthreshold dose of UVR-B. Methods: Twenty-six-week-old female albino Sprague-Dawley rats were exposed to subthreshold dose (1 kJ/m2 ) of UVR-B unilaterally and sacrificed at 1, 8, 16 and 24 hours after exposure. Lenses were enucleated and active caspase 3 was detected by western blot. The time evolution of active caspase 3 was then plotted as a function of relative mean difference in active caspase 3 between exposed and nonexposed lenses. Results: There is expression of active caspase 3 in both exposed and nonexposed lenses but there is no difference in relative mean difference in active caspase 3 between exposed and nonexposed lenses in all four postexposure groups. Conclusions: Exposure to subthreshold dose of UVR-B does not induce.. View More»