Immunome Research
Open Access
ISSN: 1745-7580
+44-77-2385-9429
ISSN: 1745-7580
+44-77-2385-9429
Charles River, Microbial Solutions, 1023 Wappoo Road, Suite 43B, Charleston, SC 29407, USA
Dr. Masakazu Tsuchiya is a Senior Research Scientist for the Microbial Solutions division of Charles River. With more than 30 years experience in the detection of endotoxin and other microbial cell wall components, notable achievements include the development of tools for microbial cell wall components, such as an endotoxin-specific Limulus Amebocyte Lysate (LAL) reagent, a beta-glucan-specific LAL reagent, and the Silkworm Larvae Plasma (SLP) reagent to detect peptidoglycan and beta-glucan.
Original Research Article
Mechanism of Low Endotoxin Recovery Caused by a Solution Containing a Chelating Agent and a Detergent
Author(s): Masakazu Tsuchiya*
Mechanism of Low Endotoxin Recovery (LER) was investigated by observing the change in particle sizes and activity of endotoxin in LER solutions containing sodium citrate and polysorbate 20. Interestingly, endotoxin aggregates were not dispersed to be smaller particles under LER conditions according to the decease of the activity. This observation was different from previous predictions of the mechanism. Endotoxin aggregates were observed by Dynamic Light Scattering, and activity was measured by the Limulus amebocyte lysate test. Particles with sizes similar to endotoxin aggregates were always observed despite different remaining activity of spiked endotoxin. This observation differed from previously proposed mechanisms for LER that dispersed endotoxin aggregates were smaller in sizes. Based on the findings, a new mechanism of LER is proposed. Purified endotoxin forms non-lamellar cubi.. View More»
DOI:
10.35248/1745-7580.19.15.166