Journal of Antivirals & Antiretrovirals
Open Access

Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda

Biography

Dr.Misaki Wayengera is working in Unit of Genetics & Genomics, Depts of Pathology & Medical Microbiology, Makerere University College of Health Sciences P O Box 7072 Kampala, Uganda. Misaki Wayengera has authored numerous research articles in well-known journals and has various other accomplishments in the related studies. Misaki Wayengera has developed valuable assistance towards the scientific community with great effort. 

Publications

• Research   
  Cloning, Expression and Purification of Recombinant Forms of Full Length and Extracellular Domain EBOV Glycoprotein within Mammalian Cell-Lines
  Author(s): Misaki Wayengera*, Peace Babirye, Carol Musubika, Samuel Kirimunda and Moses L. Joloba
  
  Background: Full length Ebolavirus glycoprotein (GP) intersperses the outer most lipid membrane to form spikes, where it mediates virus-host cell interaction. A secretory form of GP (sGP) is also produced by all 5 known Ebolavirus species. These attributes make GP an ideal target for research and development (R and D) of Ebolavirus and possibly pan-filovirus targeted Rapid Diagnostic Tests (RDTs), bio-therapeutics and vaccines. Prior cloning of recombinant Zaire ebolavirus (EBOV) GP has majorly used insect (baculovirus ) expression systems. We report the cloning, expression and purification of the full length and extracellular domain (ECD) forms of recombinant EBOV GP in mammalian cell-lines. Methods and results: 2034 and 1956 base-pair (bp) coding DNA sequences corresponding to the 669 and 643 am.. View More»
  DOI: 10.35248/1948-5964.19.11.187

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