Journal of Pharmaceutical Care & Health Systems
Open Access

Department of Geochemistry, Acharya Nagarjuna University, Guntur, India

Publications

• Research Article   
  A New Validated Rp-HPLC Method Development for the Simultaneous Estimation of Lamivudine, Tenofovir and Efavirenz in Pure and its Pharmaceutical Dosage Form
  Author(s): Nageswara Rao*
  
  This study was designed to develop a reliable method for estimation of 3TC, TFV, and EFV in pure and its pharmaceutical dosage form by RP-HPLC. Method: A simple, rapid, accurate, precise, robust and isocratic reverse phase high performance liquid chromatographic (RP-HPLC) method was developed for the estimation of 3TC, TFV and EFV in combined dosage form. The method was developed by using Inertsil ODS-3V (250 × 4.6, 5 μm) column, with Mobile phase composition of Acetonitrile: 1% IPA in the ratio of 85:15 at a flow rate of 1 ml/min and the effluents were monitored at 256 nm using PDA detector. Results: 3TC, TFV and EFV drugs were eluted at retention time of 2.4, 2.8 and 4.5 min (± 0.5). The proposed method was validated as per ICH guidelines. The correlation coefficient (R2) was found to be 0.999. All the parameters were found to be within the limits. The rec.. View More»
  DOI: 10.35248/2161-0983.22.9.251

  Abstract HTML PDF