Fungal Genomics & Biology
Open Access
ISSN: 2165-8056
ISSN: 2165-8056
Department of Botany, Baba Mastnath University, Rohtak, India
Research Article
Saccharification of Used Paper Cup for Ethanol Production by Yeast Isolate
Author(s): Navneet Kumari, Komal, SS Gulia and Kirpa Ram*
The main aim of this work was to develop an effective method for pretreatment and enzymatic saccharification
of cellulose and hemicellulose components into fermentable sugars, as well as fermentation of the hydrolysate to
bioethanol by new yeast isolates. YAJ-2M (3.2%), YCJ-1 (3.4%) and YAJ-2M 11 (5.6%) produced maximum ethanol.
UV- induction responsible for genetic improvement of yeast isolates for ethanol production. Pretreatment of paper
with alkali- acid reduced the lignin content from 25% to 14.3%. Maximum liberation of reducing sugars (168.7 μg/
ml) was attained at 50°C for 96 hours at 150 rpm using enzyme dose of 20 U/g in alkali-acid (sodium hydroxide-
orthophosphoric acid) pretreated paper cup. Fermentation of hydrolysate paper by yeast isolates (YAJ-1 M2 11
mutant) at 30°C, pH 4.7, 150 rpm and resulted in 1% ethanol after 96 h. Present investi.. View More»
DOI:
10.35841/2165-8056.22.12.185