ISSN: 2165-8048
+44 1300 500008
Department of Organic Chemistry, University of Yaounde I, Cameroon, South Africa
Review Article
Discovery of Novel Flavonoids Fractions: Based Enzymes Inhibitors of Prolyl Oligopeptidase
Author(s): Ndogo Eteme Olivier*
The following work describes the purification of recombinant expressed Prolyl Oligopeptidaseby GSH affinity capture
beads via a GST-tag and protease cleavage site. The purification steps were monitored with Commission stained
protein gel analysis. Therefore, an enzyme inhibition assay design for a well-established internally quenched
fluorophore as substrate was established. Ethylacetate (flavonoid extract), hexane and dichloromethane plant extracts
of different species of Allexis were analyzed for inhibition on POP... View More»