Journal of Alcoholism & Drug Dependence
Open Access

Department of Chemical Engineering, Kyiv National University of Technologies and Design, Kyiv, Ukraine

Publications

• Research Article   
  Influence of Nicotinamide Derivative and its Possible Metabolites on the Ethanol Metabolic Enzymes Activity
  Author(s): Olha Kyslova*
  
  Background: The aim of this investigation was to study the effect of N-phenyl-N-(1-cyclopropylethyl) nicotinamide and its possible metabolites-hydrochlorides of N-(1-cyclopropylethyl) amine and N-phenyl-N-(1-cyclopropylethyl) amine on the ethanol oxidation enzymes activity in vitro, as well as the kinetics of their interaction. Methods: The studies were performed using alcohol dehydrogenase and aldehyde dehydrogenase is enzyme forms of rat liver subcellular fractions, which were obtained by differential centrifugation. Enzyme activity was determined spectrophotometrically. The kinetic nature of enzymes interaction with nicotinamide derivative was investigated in the concentration range of 25 μM-75 μM. The obtained results were processed by the Line weaver-Burk method. Results: Studies have shown that N-phenyl.. View More»
  DOI: DOI: 10.35248/2329-6488.22.10.373

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