Enzyme Engineering
Open Access
ISSN: 2329-6674
ISSN: 2329-6674
Department of Biochemistry, Faculty of Biological Sciences, University of Nigeria, Nsukka, Enugu state, Nigeria
Research Article
Optimization of Culture Conditions for Production of β-galactosidase from Lactobacillus acidophilus Isolated from Dairy Industrial Effluent
Author(s): Oparaji EH*, Okwuenu PC, Onosakponome I, Eze SOO and Chilaka FC
β-galactosidase producing Lactobacillus was isolated from dairy industrial waste water collected from Rumuekini, Rivers state. Standard microbiology, biochemical and molecular techniques (16s DNA sequencing) were used for confirmation of the bacterial strain as Lactobacillus acidophilus. Macfarlane solution was used for standardization of the microbial inoculum and determination of the heterotrophic counts. P-NPG served as the best substrate for β- galactosidase production from Lactobacillus acidophilus with rapid yellow colouration after two days of incubation. Submerged Fermentation (SMF) system was used for the enzyme production. Inoculum size of 2 ml with total heterotrophic counts of 5.4 × 108 cfu/ml was used throughout the optimization studies. Carbon sources including: lactose, glucose, sugarcane baggase and combination of g.. View More»
DOI:
10.35841/2329-6674.22.11.1000181