Journal of Cell Signaling
Open Access
ISSN: 2576-1471
ISSN: 2576-1471
Lung and Placenta Research Laboratory, Department of Physiology and Developmental Biology, Provo,Utah, USA
Post-Doctoral Fellow in Department of Internal Medicine, Pulmonary Division, University of Utah Health Sciences Center, Salt Lake City, UT John R. and also a post-doctoral Fellow (2004-2006) Department of Internal Medicine, Pulmonary Division, University of Utah Health Sciences Center, Salt Lake City, UT 84239 John R. Hoidal, M.D., Advisor.
Research Article
The mTOR Family of Proteins and the Regulation of Trophoblast Invasion by Gas6
Author(s): Kelsey M Hirsch, Kary Ya Fang Tsai, Miki M Edward, Parker Hal, Juan F Mejia, Camilo A Meji, Paul R Reynolds and Juan A Arroyo*
Objective: Preeclampsia is an obstetric complication wherein trophoblast invasion is decreased and high levels of serum Gas6 protein are present. Our objective was to assess members of the mTOR family that are plausibly involved in the invasion of trophoblast cells resulting from Gas6/AXL activation.
Methods: Human first trimester cell line; (SW71), placental choriocarcinoma cell line (Jeg-3) and pulmonary alveolar type II-like cell line (A549) were treated with Gas6 or Gas6 and R428 (AXL receptor inhibitor) for 24 hours and real time cellular invasion was determined. Western blots were utilized to assess AXL receptor expression in treated and control cells. An Akt/mTOR phospho protein multiplex approach was used to determine the activity of mTOR related proteins.
Results: As compared to controls, cell treatments s.. View More»
DOI:
10.35248/2576-1471.19.4.203