ISSN: 0974-276X
Engineering Research Center of Agricultural Microbiology Technology, Ministry of Education, Heilongjiang University, Harbin 150080, China
Research
Membrane Proteomic Responses in Sugar Beet Roots to Salt Stress
Author(s): Jianing Kang, Peng Liao, Bing Yu, Sixue Chen, Haiying Li and Chunquan Ma*
Salt stress as one of the major abiotic stresses limits the world crop production. Sugar beet monosomic addition
line M14 is a unique germplasm with salt stress tolerance, and root is directly exposed to salt stress in the soil.
Here we report changes in root membrane proteome of the M14 plants in response to 0, 200 mM and 400
mM NaCl treatment using iTRAQ LC-MS/MS quantitative proteomics. A total of 115 differentially expressed
membrane proteins (96 increased and 19 decreased) were identified with a significant fold change of >2.0.
The proteins were mainly involved in the processes of transport, signaling, stress and defense, energy, protein
degradation, and transcription. Transcriptional changes of 10 genes encoding the differential membrane proteins
were analyzed using real-time PCR, and seven genes showed a positive correlation between transcriptional and
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DOI:
10.35248/0974-276X.21.14.541