ISSN: 2329-9029
+44 1478 350008
Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Gonawila Makandura, Gonawila 60170, Sri Lanka
Dr. Prasanthi I P Perera is a Senior lecturer at the Wayamba University of Sri Lanka and Faculty of Agriculture and Plantation Management, Department of Horticulture and Landscape Gardening, Sri Lanka. She had published 21 articles which are quite informative and interesting. She had worked on a project on Plant genetic resources, Scientific Editing, etc.
Research Article
In vitro Culture of Heat-treated Anthers Induces Embryogenic Callus in Cassava (Manihot esculenta Crantz)
Author(s): Buttibwa M, Kawuki RS, Oshaba B, Eyokia M, Hershey C, Perera PIP, Heberle-Bors E, Baguma Y and Tugume AK*
Microspore embryogenesis is the developmental plasticity of juvenile male gametophytes to switch from pollen to embryo development upon exposure to in vitro stress. It is a common method for obtaining haploid and doubled haploid plants in breeding programs for development of superior varieties via haploid-diploidization which allows fast development of homozygous lines from heterozygous parents. In cassava, obtaining haploidization through traditional methods of successive selfing is difficult because of cassava’s long reproductive cycle, high heterozygosity, and inbreeding depression. As a first step towards microspore embryogenesis, callus induction following heat treatment has not been investigated in cassava. We used two elite Ugandan cassava varieties, hereafter called “genotypes”, NASE3 and NASE14, for the study of callus induction. Heat stress of 40°C for .. View More»
DOI:
10.35248/2329-9029.20.8.249