Journal of Plant Biochemistry & Physiology
Open Access

Faculty of Agriculture and Plantation Management, Wayamba University of Sri Lanka, Gonawila Makandura, Gonawila 60170, Sri Lanka

Publications

• Research Article   
  In vitro Culture of Heat-treated Anthers Induces Embryogenic Callus in Cassava (Manihot esculenta Crantz)
  Author(s): Buttibwa M, Kawuki RS, Oshaba B, Eyokia M, Hershey C, Perera PIP, Heberle-Bors E, Baguma Y and Tugume AK*
  
  Microspore embryogenesis is the developmental plasticity of juvenile male gametophytes to switch from pollen to embryo development upon exposure to in vitro stress. It is a common method for obtaining haploid and doubled haploid plants in breeding programs for development of superior varieties via haploid-diploidization which allows fast development of homozygous lines from heterozygous parents. In cassava, obtaining haploidization through traditional methods of successive selfing is difficult because of cassava’s long reproductive cycle, high heterozygosity, and inbreeding depression. As a first step towards microspore embryogenesis, callus induction following heat treatment has not been investigated in cassava. We used two elite Ugandan cassava varieties, hereafter called “genotypes”, NASE3 and NASE14, for the study of callus induction. Heat stress of 40°C for .. View More»
  DOI: 10.35248/2329-9029.20.8.249

  Abstract HTML PDF