Applied Microbiology: Open Access
Open Access

Department of Bioengineering, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China

Publications

• Mini Review   
  The Application of CRISPR/Cas9 System in the Basidiomycete Ganoderma lucidum
  Author(s): Ping-An Wang*
  
  Ganoderma lucidum, a kind of basidiomycete, can produce various valuable metabolites with bioactive. For a long time, the shortage of the genome and gene manipulation methods limited the analysis of gene functions, which delayed the elucidation of biosynthesis pathways of those products in G. lucidum. As G. lucidum was sequenced and the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein-9 nuclease (Cas9) system was established, gene modification and gene function analysis was feasible. In this review, we summarized the establishment of CRISPR/Cas9 system and its biotechnological application in G. lucidum. Besides, we discuss challenges to the current CRISPR/Cas9 system and give perspectives on the biotechnology research in G. lucidum... View More»
  DOI: 10.35284/2471-9315.23.9.270

  Abstract HTML PDF