Immunotherapy: Open Access
Open Access
ISSN: 2471-9552
ISSN: 2471-9552
Department of Respiratory and Critical Care Medicine, Tianjin Medical University General Hospital, Tianjin 300052, China
Research
The Effect of Histone Deacetylase Inhibitor Trichostatin A on IL-
17A-Induced Transformation of MRC5 into Myofibroblasts and its
Mechanism
Author(s): Qian Zhang, Zexin Guo, Jing Zhang, Wei Zhou, Yueqing Wu, Lixia Dong, Jing Feng, Shuo Li* and Jie Cao*
Objective: To elucidate potential IL-17A- and TSA-mediated regulation of fibroblasts transformation.
Method: MTT assay, HDAC1 activity assay, cell immunofluorescence and Western blot were employed to detect the
expression of related indicators.
Results: MRC5 cells expressed only a small amount of Vimentin. IL-17A treatment up regulated MRC5 cell
proliferation, in a concentration-dependent manner. TSA treatment, however, suppressed MRC5 cell proliferation.
IL-17A treatment also upregulated HDAC1 activity in MRC5 cells, in a concentration-dependent manner. Using
immunofluorescence, we demonstrated that IL-17A-treated MRC5 cells had markedly elevated Vimentin, Collagen-I
and a-SMA levels, compared to controls. However, a combined treatment of IL-17A and TSA resulted in markedly
reduced levels of the Vime.. View More»