Journal of Clinical Chemistry and Laboratory Medicine
Open Access

Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, Italy

Biography

Roberto Pellegrino has been employed as a technician at the Department of Chemistry, Biology and Biotechnology of the University of Perugia since 1987 where he’s dealt with instrumental analytical chemistry, with particular reference to chromatographic techniques and mass spectrometry. He’s a specialist in Analytical Chemistry, Mass Spectrometry, Metabolomics and Lipidomics

Publications

• Research Article   
  Validation of Immunoblot SDS-page as a Qualitative and a Quantitative Method for Determination of Urinary Cystatin C in Neonates
  Author(s): Chiara Grasselli*, A. Barbati, L. Cesarini, R. Pellegrino and G.C. Di Renzo
  
  Objective: The measurement of urinary Cystatin C (cyst-C) can be a very good way for early identifying newborns predisposed to renal function impairment. PETIA, PENIA and EIA are the immunometric methods used for quantitative determination of cyst-C in human biologic fluid but they have some interference and do not allow realizing qualitative analysis. The present study performs a validation of Immunoblot SDS-PAGE for the analysis of urinary cyst-C. Methods: urines were collected from neonates of the nursey at S. Maria della Misericordia Hospital. Urinary cyst-C was investigated by the SDS-PAGE electrophoresis, the immunoblot and the reading of optical density. Results: the qualitative analysis showed two different molecular forms: a reactivity at about 70 KDa in all samples and a reactivity a.. View More»
  DOI: 10.35248/jcclm.20.3.141

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