Journal of Clinical Chemistry and Laboratory Medicine
Open Access

Department of Chemistry, Biology and Biotechnology, University of Perugia, Perugia, Italy

Publications

• Research Article   
  Validation of Immunoblot SDS-page as a Qualitative and a Quantitative Method for Determination of Urinary Cystatin C in Neonates
  Author(s): Chiara Grasselli*, A. Barbati, L. Cesarini, R. Pellegrino and G.C. Di Renzo
  
  Objective: The measurement of urinary Cystatin C (cyst-C) can be a very good way for early identifying newborns predisposed to renal function impairment. PETIA, PENIA and EIA are the immunometric methods used for quantitative determination of cyst-C in human biologic fluid but they have some interference and do not allow realizing qualitative analysis. The present study performs a validation of Immunoblot SDS-PAGE for the analysis of urinary cyst-C. Methods: urines were collected from neonates of the nursey at S. Maria della Misericordia Hospital. Urinary cyst-C was investigated by the SDS-PAGE electrophoresis, the immunoblot and the reading of optical density. Results: the qualitative analysis showed two different molecular forms: a reactivity at about 70 KDa in all samples and a reactivity a.. View More»
  DOI: 10.35248/jcclm.20.3.141

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