Cloning & Transgenesis
Open Access
ISSN: 2168-9849
ISSN: 2168-9849
Department of genetics, Akbarabadi Hospital, Iran University of Medical Sciences, Tehran, Iran, Iran
Research Article
Cloning of Streptokinase Gene under Thermal Sensitive Promoter of Lambda Phage into E. coli Genome
Author(s): Rahman Yavar*
Bacterial cell culture is superior to using a cheap culture medium and rapid cell growth at high concentrations. High concentrations of cells increase the rate of protein production in the bacterial expression system, resulting in high volumes of recombinant protein. E.coli is the most common host and the first choice for making many recombinant proteins. One of the most important substances in protein production is the expression carrier properties used. Having a good and adjustable promoter in the cloned gene is one of the most important control characteristics for the gene host... View More»