Immunotherapy: Open Access
Open Access

Institute of Cancer Sciences, University of Manchester, Manchester, UK

Biography

After general medical training, including an intercalated BSc in Anatomy and MSc in Cell and Structural Biology, I trained as a histopathologist and did a PhD in molecular biology, specifically in bone biology. Since then I have developed a diagnostic and research interest in haematopathology and my specialist diagnostic clinical interest is in leukaemia and lymphoma. I am Reader in Pathology at the University of Manchester and Consultant Haematopathologist at Manchester Royal Infirmary. 

Publications

• Research   
  Multiplex Immunohistochemistry: The Importance of Staining Order When Producing a Validated Protocol
  Author(s): Jihad Syed, Jack Ashton, Jesuchristopher Joseph, Gemma N Jones, Christian Slater, Alan Sharpe, Garry Ashton, William Howat, Richard Byers and Helen K Angell*
  
  Aim: The complexity of multifactorial diseases, such as cancer, poses significant challenges to the development of personalised therapies. Integrated digital histological analysis of tumours provides a better understanding of the immune microenvironment and the prognostic relationship associated with the enumeration and distribution of specific tumour infiltrating lymphocyte (TILs) subpopulations. To this effect multiplex cell labelling , alongside multi-spectral imaging (MSI) is an approach increasingly used to achieve more accurate in-situ TIL phenotyping and quantification. However, these approaches require full validation prior to utilisation , which is the fundamental aim of this study. Methods: Whole sections and tissue microarrays of lymphocyte-rich tissue were used to develop and validate a multiplex immunofluorescence (IF) protocol .. View More»
  

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